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reverse pipetting
Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: as it reduces the risk of splashing, foam or bubble formation. Reverse pipetting is more precise in dispensing small volumes of liquids containing proteins and biological solutions compared to forward pipetting, which is mostly used for aqueous solutions, such as buffers, diluted acids or alkalis.
== How it works ==
(詳細はair displacement pipette, the piston inside the instrument moves down to let air out. Air is displaced by the piston. The volume of air displaced is equivalent to the volume of liquid aspirated.
These pipettes are capable of being very precise and accurate. However, since they rely on air displacement, they are subject to inaccuracies caused by the changing environment, particularly temperature and user technique. For these reasons this equipment must be carefully maintained and calibrated, and users must be trained to exercise correct and consistent technique.
Alternative solutions to improve reproducibility and accuracy of manual pipetting operations are based on anthropomorphic liquid handling robots capable to handle air displacement pipettes.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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